Determination of long-chain base in glycosphingolipids with fluorescamine
نویسندگان
چکیده
منابع مشابه
Determination of long-chain base in glycosphingolipids with fluorescamine.
A method is described for determining the long-chain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.
متن کاملLong-chain fatty acid determination
You, J., X. Zhao, Y. Suo, Y. Li, H. Wang, and G. Chen, Determination of LongChain Fatty Acids in Bryophyte Plant Extracts by HPLC with Fluorescence Detection and Identification with MS, J. Chromatogr. B 848:283–291, 2007. A sensitive method has been developed for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(ptoluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phena...
متن کاملDetermination of ganglioside non-hydroxy fatty acid and long chain base by analysis of perbenzoylated derivatives.
The non-hydroxy fatty acid and long chain base compositions from as little as 2.7 nmol of ganglioside were ascertained from perbenzoylated ganglioside derivatives. Non-hydroxy fatty acids were determined by mild alkaline methanolysis of the derivatives, followed by gas-liquid chromatography (GLC) of the methyl esters. N-acyl and N-benzoyl "gangliosides" that were generated by the methanolysis w...
متن کاملCharacterization of sphingolipid long-chain base kinase in Arabidopsis thaliana.
Sphingolipid long-chain base (LCB) kinase catalyses the phosphorylation of sphingolipid LCB to form LCB 1-phosphate. Based on sequence identity to a murine sphingosine kinase (murine SPHK1a), we isolated and characterized a LCB kinase-like cDNA in Arabidopsis thaliana. The deduced amino acid sequence of the homologous cDNA shows several regions that are highly conserved in LCB kinases from mous...
متن کاملdetermination of maximal singularity free zones in the workspace of parallel manipulator
due to the limiting workspace of parallel manipulator and regarding to finding the trajectory planning of singularity free at workspace is difficult, so finding a best solution that can develop a technique to determine the singularity-free zones in the workspace of parallel manipulators is highly important. in this thesis a simple and new technique are presented to determine the maximal singula...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1974
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)36822-x